EFFECT OF HYDRAZINES ON VITAMIN B6 LEVELS IN THE MOUSE BRAIN.
Final rept., 1 Mar 65-28 Feb 66,
SAN FRANCISCO UNIV CALIF INST OF CHEMICAL BIOLOGY
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The effects of administered 1,1-dimethylhydrazine UDMH and monomethylhydrazine MMH on vitamin B6 levels in mouse brain have been studied. Separation of the B6 group pyridoxol, pyridoxal, pyridoxamine, and the respective 5-phosphates by means of paper chromatography revealed that the R sub F values obtained are dependent upon the pH of the developing solvent. To obtain the time lag just prior to convulsions induced by UDMH and MMH, a dose-lag time study was conducted included were pyridoxal and its 5-phosphate hydrazones of UDMH and MMH. Graphs of log dose vs lag time are given. The bioassay procedure, though not completed, permits detection of some of the B6 congeners to a limit of 0.5 nanograms. Author