Accession Number:

AD0647192

Title:

EFFECT OF HYDRAZINES ON VITAMIN B6 LEVELS IN THE MOUSE BRAIN.

Descriptive Note:

Final rept., 1 Mar 65-28 Feb 66,

Corporate Author:

SAN FRANCISCO UNIV CALIF INST OF CHEMICAL BIOLOGY

Personal Author(s):

• Furst,Arthur
• Gustavson,Waldemar R.

Report Date:

1966-09-01

Pagination or Media Count:

46.0

Abstract:

The effects of administered 1,1-dimethylhydrazine UDMH and monomethylhydrazine MMH on vitamin B6 levels in mouse brain have been studied. Separation of the B6 group pyridoxol, pyridoxal, pyridoxamine, and the respective 5-phosphates by means of paper chromatography revealed that the R sub F values obtained are dependent upon the pH of the developing solvent. To obtain the time lag just prior to convulsions induced by UDMH and MMH, a dose-lag time study was conducted included were pyridoxal and its 5-phosphate hydrazones of UDMH and MMH. Graphs of log dose vs lag time are given. The bioassay procedure, though not completed, permits detection of some of the B6 congeners to a limit of 0.5 nanograms. Author

Descriptors:

• TOXICITY
• VITAMIN B COMPLEX
• DIMETHYLHYDRAZINES
• BIOASSAY
• MICE
• BRAIN
• CHROMATOGRAPHY
• PH FACTOR
• DOSAGE
• YEASTS
• CULTURE MEDIA

Subject Categories:

• Biochemistry
• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE