REACTIVATION BY 2-PAMCL OF INHIBITED CHE ACTIVITY IN DOGS POISONED WITH PINACOLYL METHYLPHOSPHONOFLUORIDATE (SOMAN, GD)
Rept. for Apr 1964-May 1965
EDGEWOOD ARSENAL ABERDEEN PROVING GROUND MD
Pagination or Media Count:
The effect of varying concentrations and dosages of 2-pyridine aldoxime methochloride 2-PAMCl on the aging of cholinesterase ChE phosphonylated by soman in vitro and in vivo in dogs was studied. The rate of aging of dog red-blood-cell RBC ChE inhibited by soman in vitro and in vivo can be markedly diminished by 2-PAMCl, which reactivates the unaged portion of the inhibited ChE enzyme. Measurements of brain- and diaphragm-ChE activity in dogs poisoned with soman and treated with 0.104 gmkg of 2-PAMCl showed that reactivation occurred only in the latter tissue. The close correlation found between in vitro and in vivo rates of aging suggests that, in a given species, estimation of the rate of aging of RBC ChE in vitro following inhibition with an organophosphate could determine the time during which oxime therapy would be effective in vivo against intoxication by the same anti-ChE.
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