EFFECTS OF RADIO-FREQUENCY IRRADIATION ON THE ENZYMES OF BEEF MUSCLE TISSUE
Technical Report,13 Aug 1964,13 Dec 1964
MELPAR FALLS CHURCH VA FALLS CHURCH
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The objective of this program is to study the conditions necessary for maximum inactivation of the natural proteolytic enzymes of beef muscle tissue by radio-frequency radiation. Achievement of this objective may contribute to the successful storage of beef under non-refrigerated conditions after its sterilization by high energy ionizing radiation. Since little is known about the mechanism of inactivation of enzyme by r-f energy, the problem was approached empirically by observing effects at the most probable conditions. Effort has been concentrated on determination of the conditions necessary for the successful inactivation of the enzyme. Three factorial designs covering as many as five variables have been devised and tested. These designs were analyzed by the conventional Yates Algorithm method. Parameters studied were frequency, temperature, voltage power, duty cycle and irradiation time. Other parameters which might have an effect on the denaturation process were kept constant within the design. From the three design studies it can not be conclusively determined what the main factors and their interaction factors are. However, at specified conditions of an experiment, it was possible to obtain as much as 40 percent deactivation of the proteolytic enzyme. Optimization of parameters may result in obtaining greater deactivation of enzyme.