EFFECTS OF RADIO-FREQUENCY IRRADIATIONS ON THE ENZYMES OF BEEF MUSCLE TISSUE
Technical Report,13 Apr 1964,13 Aug 1964
MELPAR FALLS CHURCH VA FALLS CHURCH
Pagination or Media Count:
The natural proteolytic enzyme of beef muscle has been extracted and purified, using procedures analogous to those described in the literature for isolation of proteolytic activity from rabbit muscle. The activity of the enzyme has been differentiated from that of pepsin and trypsin, and some of its characteristics have been established. Appropriate assay procedures have been developed based on the hydrolyses of urea denatured hemoglobin as measured by increase in optical absorbance at 280 millimicrons. Active purified preparations of the proteolytic enzyme have been subjected to radio-frequency irradiation over a limited range in beginning to determine the correct frequency at which the enzyme is most susceptible to attack in vitro. Differences between the activity of irradiated and nonirradiated aliquots of the enzyme are determined by appropriate statistical procedures.
- Food, Food Service and Nutrition