Accession Number:

AD0644218

Title:

THE ISOLATION OF CRYSTALLINE I-UROBILINOGEN (MESOBILIRUBINOGEN) FROM HUMAN FECES AND RESOLUTION OF THE CORRESPONDING UROBILIN,

Descriptive Note:

Corporate Author:

MINNESOTA UNIV MINNEAPOLIS

Report Date:

1965-01-01

Pagination or Media Count:

1.0

Abstract:

Crystalline i-urobilinogen was isolated from human feces obtained 5-6 days after discontinuance of tetracyclin. The method of isolation was essentially that previously described for d-urobilinogen J. Biol. Chem. 218641, 1956. The natural material was identical with synthetic mesobilirubinogen as regards melting point, mixed melting point, crystal habit and x-ray crystal powder patterns. Both were optically inactive throughout the range of 600-200 millimicrons. The corresponding i-urobilin, prepared from 4 x recrystallized natural chromogen, has been resolved by fractional crystallization a d-U crystallizes out Specific rotation 20C, D line 5280 degrees. The results of FeCl3 oxidation J. Biol. Chem. 235787, 1960 were typical of a pure d-U. The mother liquor was levorotatory, Specific rotation 20C, D line -1870 degrees related to optical density at 490 millimicrons. Partial resolution of synthetic i-U into strongly positively and negatively rotating forms has also been achieved. d-Urobilinogen H44 has been crystallized following catalytic hydrogenation of d-U H40. The crystals differed from the natural or synthetic mesobilirubinogen in crystal habit, x-ray crystal powder pattern, and ORD curve. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE