Accession Number:

AD0642071

Title:

INFLUENCE OF THE IONS OF TRIVALENT IRON ON THE PHYSICAL-CHEMICAL PROPERTIES OF SOLUTIONS OF NATIVE DESOXYRIBONUCLEIC ACID

Descriptive Note:

Journal article

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Report Date:

1966-01-01

Pagination or Media Count:

12.0

Abstract:

An investigation was made on the influence of the addition of Fe3 on the viscosity, dispersion curves of optical rotation and the absorption spectra of solutions of native DNA. It was shown that with the formation of a complex of DNA with Fe3 a lessening takes place in the values of the quoted viscosity in a wide range of concentrations of DNA. In the presence of Fe3 ions an increase is observed in the value of the specific rotation and a decrease in the absorption coefficient of solutions of DNA, and also an increase of the melting temperature in comparison with the melting temperature of pure DNA. The experimental data obtained agree with the model of a macromolecule of DNA, having a certain number of points at which there is the possibility for the rotation of parts of the molecule which are stable rods. The addition of Fe3 ions leads to an increase in the rigidity of the DNA macromolecule, by reducing the number of places around which a bending of the macromolecule takes place.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE