Accession Number:

AD0641910

Title:

AN ABSORPTION SPECTROPHOTOMETER CELL FOR USE AT HIGH PRESSURES AND ITS APPLICATION TO AN ENZYMIC REACTION.

Descriptive Note:

Research rept.,

Corporate Author:

NAVAL MEDICAL RESEARCH INST BETHESDA MD

Personal Author(s):

• Steiner,Robert F.

Report Date:

1966-11-07

Pagination or Media Count:

16.0

Abstract:

A cell designed for absorbancy measurements at high pressures has been constructed and used for a study of the pressure dependence of a thrombincatalyzed hydrolysis. Thrombin is not inactivated at pressures up to 20,000 psi. The rate of hydrolysis of a synthetic substrate toluene sulfonyl arginine methyl ester increases appreciably with pressure. Author

Descriptors:

• (*SPECTROPHOTOMETERS
• CONTAINERS)
• (*ENZYMES
• HIGH PRESSURE)
• (*ULTRAVIOLET SPECTROSCOPY
• INSTRUMENTATION)
• ABSORPTION SPECTRA
• OPTICAL EQUIPMENT COMPONENTS
• HYDROLYSIS
• CATALYSIS
• THROMBIN
• REACTION KINETICS
• DEEP SUBMERGENCE

Subject Categories:

• Biochemistry
• Medical Facilities, Equipment and Supplies
• Test Facilities, Equipment and Methods

Distribution Statement:

APPROVED FOR PUBLIC RELEASE