Accession Number:

AD0639641

Title:

CHEMICAL PROTECTION AGAINST RADIATION DAMAGE TO AN IMMUNE RESPONSE IN MOUSE SPLEEN CELLS IN VITRO.

Descriptive Note:

Rept. for 1 Sep 65-28 Feb 66,

Corporate Author:

ALBERT EINSTEIN MEDICAL CENTER PHILADELPHIA PA

Report Date:

1966-08-01

Pagination or Media Count:

13.0

Abstract:

Swiss Webster mice were sensitized by injection of sheep red blood cells and were sacrificed 4 days later. Suspensions of teased spleen cells were made and irradiated in the presence of MEG, GED, ascorbic acid, dehydroascorbic acid, or no agent. The cells were incubated after plating with agar, sheep red cells, dextran, and complement, and hemolytic plaques were counted. Plaques represent spleen cells which are still able to release antibody. Radiation reduced the number of plaques formed with a D37 of 8 to 16 kR. MEG, GED, and dehydroascorbic acid did not protect. Ascorbic acid showed good protection with D37 44, 33, 50 in three separate runs. The mechanism of this protection is under study. Author

Subject Categories:

  • Medicine and Medical Research
  • Radiobiology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE