EVIDENCE FOR PLURIPOTENTIALITY OF MARROW STEM CELLS: MODIFICATION OF TISSUE DISTRIBUTION OF IN VIVO I125-UDR LABELED TRANSPLANTED MARROW.
NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF
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Mice were exposed to a lethal dose of X-radiation 900 rad and transfused with 5,000,000 syngeneic bone marrow cells. Subgroups of these mice were either made polycythemic, anemic or served as controls. Regenerative activity in the hemopoietic and lymphopoietic tissues was assessed by flash-labeling with I125-iododeoxyuridine. The evidence based on the incorporation of I125-labeled iododeoxyuridine into DNA, organ weights, histological and H3-thymidine rediautographic data suggests that stem cells in the bone marrow of adult mice may contain both hemopoietic and lympho-plasmacytopoietic potentialities. The methodology and some possible objections to the interpretation of the data are discussed, and future work is indicated. Author