MORPHOLOGIC AND IMMUNOHISTOCHEMICAL STUDIES OF THE PATHOGENESIS OF INFECTION AND ANTIBODY FORMATION SUBSEQUENT TO VACCINATION OF MACACA IRUS WITH AN ATTENUATED STRAIN OF PASTEURELLA TULARENSIS. II. AEROGENIC VACCINATION
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Twenty-four cynomolgus monkeys were vaccinated aerogenically with the living vaccine strain LVS of P. tularensis. The average inhaled dose was 270, 000 viable cells. The bacteria initiated a mild, nongranulomatous inflammatory response in the respiratory bronchioles that was completely resolved by the 14th day after vaccination. LVS disseminated to involve the intrapulmonic lymphoid tissues, the tracheobronchial lymph nodes, the liver and the spleen. By the 28th day all sites except the tracheobronchial lymph nodes were sterile, and no bacteria were recovered from these nodes on the 90th day. Anti-tularensis gamma globulin ATGG appeared in plasma cell precursors in the lung, about respiratory bronchioles, and in the peribronchial lymphoid tissues by the seventh day. By the 14th day mature plasma cells containing ATGG were prominent. The appearance of ATGG in the regional lymph nodes and spleen was as prompt as that found in the dermal vaccinees.