RED BLOOD CELL PRESERVATION.
Progress rept. for 1 Jul 65-30 Jun 66,
VIRGINIA UNIV CHARLOTTESVILLE BIOCHEMICAL LAB
Pagination or Media Count:
A number of erythrocyte phosphate metabolites adenosine tri- and diphosphates, guanosine triphosphate, 2,3-diphosphoglycerate affected heme-heme interaction and oxygen affinity with hemoglobin which resulted in a shift of the oxyhemoglobin dissociation curve to the right. In addition, tripolyphosphate, and tetra- and hexametaphosphates, particularly the latter, affected the dissociation curve in the same manner. Adenosine monophosphate and ribose-5-phosphate had no effect. These results, which are preliminary, were obtained with hemolysates and purified hemoglobin. Bloods, collected in ACD pH 5.0 and CPD pH 5.75 with and without additives, were stored in the cold 4C for six weeks. After 0,2,4 and 6 weeks storage, the erythrocytes were assayed for DPG, ATP, ADP, AMP, IMP, TPN and GTP by ion exchange chromatography. The ATP of red cells in stored unsupplemented blood was maintained at higher concentrations than those stored in CPD blood DPG levels were greater in CPD blood. These differences were due to the pH of the blood. The remaining compounds were not appreciably changed during storage. In ACD blood supplemented with small amounts of adenine 0.05 to 0.10 mmole100 ml, the ATP concentrations were higher than in those which contained greater amounts of adenine 0.5 mmole. The DPG values decreased rapidly in these bloods. Author