ENZYME HISTOCHEMISTRY OF ACUTE STAPHYLOCOCCAL ENTEROTOXIN GASTROENTERITIS IN RHESUS MONKEYS
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC
Pagination or Media Count:
Rhesus monkeys given 150 micrograms of purified staphylococcal enterotoxin B developed acute gastroenteritis. This was well developed at 2 hours, maximal at 4 to 8 hours and rapidly regressed 12 to 72 hours after enterotoxin administration. These features were compared with changes in enzyme activities acid and alkaline phosphatase, glucose-6 -phosphatase, DPNH and TPNH diaphorases, succinic dehydrogenase, glucose-6-phosphate dehydrogenase and the hexokinase system. In the gastric mucosa at the height of the lesion there was a slight reduction of enzyme activity in surface mucous epithelium and a slight increase in acid phosphatase activity in chief cells. No change was demonstrated in parietal cell enzymes. In jejunal epithelium a rapid decrease and recovery of enzyme activity was noted. This correlated well with alterations in the structure of the epithelium. In the ileum, where epithelial alterations were not conspicuous, changes in enzyme activity were slight. Lipid droplets disappeared from the jejunal mucosa and appeared in the ileal mucosa at the height of the lesion suggesting an alteration in the pattern of fat absorption.
- Medicine and Medical Research