Accession Number:

AD0631145

Title:

THE DNA CONTENT OF MITOTIC NUCLEI OF TWO MOUSE TUMORS,

Descriptive Note:

Corporate Author:

NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s):

Report Date:

1966-03-03

Pagination or Media Count:

18.0

Abstract:

A new high-precision microspectrophotometric method was used to test the validity of the DNA constancy hypothesis in two mammalian tumor lines. The two are the mouse ascitic tumor BP-8 and the in vitro mouse leukemic cell line, L5178Y. It was shown that the mitotic nuclei of these cells have a DNA content with a coefficient of variation of 5-6. The instrumental and measurement errors are 4-5 thus the variability of DNA content as measured by absorption of Feulgen stain is 1 or less. Author

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Radiobiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE