Accession Number:

AD0631144

Title:

RADIATION-INDUCED FREE POLYDEOXYRIBONUCLEOTIDES IN LYMPHOID TISSUES: A PRODUCT OF THE ACTION OF DEOXYRIBONUCLEASE I,

Descriptive Note:

Corporate Author:

NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s):

Report Date:

1966-03-01

Pagination or Media Count:

24.0

Abstract:

Physico-chemical studies on the saline-extractable polydeoxyribonucleotides PDN occurring in lymphoid tissues 4 hours after whole-body exposure to 600 rads X-irradiation revealed the following information 1 Viscosity, precipitability by acids and alcohols, and behavior on gel-filtration columns suggest a molecular weight less than that of undegraded DNA but probably greater than 100000. 2 Thermal transition curves indicate extensive complementary double stranding with slight fraying of the ends of the strands. 3 In 0.2 M NaCl, PDN has a lower affinity for histone than does DNA. 4 Susceptibility of PDN to the action of venom phosphodiesterase EC 3.1.4.1 and resistance to spleen phosphodiesterase EC 3.1.4.1 demonstrate the presence of 3-hydroxyl terminals and the absence of 5-hydroxyl terminals. 5 The above observations suggest that PDN was formed from DNA by the action of a deoxyribonuclease I-like EC 3.1.4.5 endonuclease rather than a deoxyribonuclease II-like EC 3.1.5.6 endonuclease in the irradiated tissue. Author

Subject Categories:

  • Radiobiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE