THE METABOLIC ACTIVITY OF INTACT CELL DISPERSATES FROM NORMAL AND OXYGEN INTOXICATED RATS.
Final rept. 1 Feb 63-30 Jun 64,
MIAMI VALLEY HOSPITAL DAYTON OHIO DEPT OF RESEARCH
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Methodology for the preparation of intact tissue dispersates was investigated. The metabolic behavior of liver cell dispersates was examined and an assay system suitable to evaluate metabolic activity effected. Metabolic alterations induced in animals exposed to 98 - 99 O2 at 13 and at 1 atmosphere have been investigated. Intact liver cell dispersates derived from rats exposed to O2 environments 1 atmosphere for periods up to death show 40 greater respiratory activity than control rats. In addition, the livers from these O2 intoxicated animals showed decreased mass, decreased cell size, and an increase in total nitrogen. These effects were not time dependent. The only effect noted in animals exposed at 13 atmosphere of 98 - 99 O2 was a decrease in liver mass. The significance of these results in O2 intoxication is discussed. Author