Accession Number:

AD0630331

Title:

THE INCORPORATION OF TRITIUM FROM THYMIDINE INTO PROTEINS OF THE MOUSE,

Descriptive Note:

Corporate Author:

NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s):

Report Date:

1966-01-21

Pagination or Media Count:

26.0

Abstract:

Tritium from methyl-3H-Thymidine was found to be incorporated into proteins in mice. This incorporation in the mouse as a whole represented between 1 and 10 of the injected tritium. Tritiated water was not responsible for the labeling. Transmethylation reactions were proposed as a means whereby certain amino acids might have acquired the tritium from thymidine at some stage of its catabolysis. The initial 2-hour ratios of DNA to protein tritium activities per mg wet tissue ranged from 5 in two tissues of low DNA synthetic activity pancreas, liver to 35-40 in two tissues of high DNA synthetic activity spleen, small intestine. Labeled nuclear protein was coincident with labeded DNA in nuclei, where it constituted less than 2.5 of the total tritium. The significance of the findings was discussed. Author

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE