STUDIES OF THE IMMUNOLOGICAL FACTORS INVOLVED IN HOMOLOGOUS TISSUE TRANSPLANTATION.
Annual rept., 1 Mar 65-28 Feb 66,
WESTERN RESERVE UNIV CLEVELAND OHIO SCHOOL OF MEDICINE
Pagination or Media Count:
A symbolic representation of amino acid side-chains is suggested which emphasizes the distribution of sites for weak intermolecular interactions. A routine has been established for the examination of cultures of leukocytes from different individuals. As has been shown elsewhere, mixed cultures show a stimulated incorporation of tritiated thymidine. The system will be used to study the matching problem in the selection of donors of transplanted tissues. Conditions have been established for the in vitro labeling of rat peripheral leukocytes with tritiated leucine. The label was confined to the protein fraction of the cells. The combining site -containing fragment of rabbit antibody has been treated with dinitrofluorobenzene. The combining site-containing fragment of rabbit antibody has been treated with dinitrofluorobenzene. The sulfhydryl groups and the amino groups are substituted and may be determined in the presence of each other by a spectro-photometric method. Experiments are outlined for the degradation of the derivative into peptides in order to partially test a theory in which heterogeneity of antibody is said to be an absolute requirement for specificity. The rate of disappearance from a skin site of a labeled antigen in delayed hypersensitive animals was ascertained. In a group of animals displaying Arthus sensitivity, the specific antigen was removed at an accelerated rate.
- Medicine and Medical Research