CYTOTOXICITY OF HUMAN SERA IN TISSUE CULTURE SYSTEMS
NAVAL MEDICAL RESEARCH UNIT NO 4 GREAT LAKES IL
Pagination or Media Count:
While performing adenovirus neutralization tests in micro-titer plates, 148 84 of 176 heat-inactivated human sera exhibited cytotoxicity for HEp-2 cells at a 12 dilution. Titers ranged from 12 to 1128 in well and ill individuals. Curtain electrophoretic analysis indicated that toxic factor moved with the prealbumin fraction. Presence in prealbumin was confirmed by Ouchterlony tests against prealbumin antisera and by disc electrophoresis methods. For further confirmation, 15 sera from three persons with varying levels of cytotoxicity for HeLa, HEp-2, KB, and WI38 cell lines were treated by ammonium sulfate precipitation, and two other highly toxic sera were submitted to curtain electrophoresis. The fractions were then tested for cytotoxicity in HeLa and HEp-2 cell lines. Toxicity was not demonstrated by any of the globulins that were separated from the 17 sera. It was concluded that the toxic substance was a prealbumin possibly associated with some physiological change in the human body.
- Anatomy and Physiology