THE EFFECT OF HYPERBARIC OXYGEN ON MICROORGANISMS IN VITRO AND IN VIVO
CORNELL UNIV MEDICAL COLL (WEILL) NEW YORK NEW YORK
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The studies indicate that oxygen is effective in killing C. perfringens and C. tetani in vitro. However, oxygen under pressure at 30 p.s.i.a. is no more bactericidal than air at atmospheric pressure 15 p.s.i.a.. Furthermore, blood and muscle markedly inhibit the anticlostridial effect of oxygen. Protection of mice against death from infection with C. perfringens can be demonstrated only if the model is carefully chosen. Oxygen will not protect mice infected by intramuscular injection in normal muscle of C. perfringens, C. perfringens with calcium chloride, or C. perfringens with infusorial earth. Nor was protection observed when mice were infected by intramuscular injection of C. perfringens or C. perfringens with calcium chloride into crushed muscle. Protection by oxygen was observed only when mice were infected by injection of C. perfringens with infusorial earth into crushed muscle. Oxygen at 30 p.s.i.a. was inhibitory for some aerobic bacteria including strains of S. typhimurium, D. pneumoniae and S. aureus. However, mortality rates were not altered by exposure of mice to oxygen at 45 p.s.i.a. following intravenous injection of S. typhimurium, D. pneumoniae or S. aureus. Whereas no protection was afforded by oxygen therapy, it is also of significance that no increase in mortality from infection occurred in mice exposed to oxygen.