DEVELOPMENT OF THEORY TO UNIFY MECHANISMS OF ENZYME CATALYSIS AND DENATURATION.
Final rept., 1 Jul 64-30 Jun 65,
NORTH SHORE HOSPITAL MANHASSET N Y
Pagination or Media Count:
Study of the denaturation of prostatic acid phosphatase was continued. Denaturation of the enzyme with ultraviolet radiation over a range of carefully controlled temperatures from -30 to 50C. was carried out in specially designed apparatus. The denaturation rate was also measured using urea or n-propanol in combination with ultraviolet radiation. Comparison was made with rates obtained using urea with the addition of other chemical agents already investigated guanidine-hydrochloride, sodium p-toluene sulfonate, and n-propanol. The effect on the denaturation rate of acid phosphatase was also measured using two series of chemical compounds chlorides of the alkali metals and methylated urea compounds symmetrical dimethyl urea and tetramethyl urea. Author