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BIOLOGICAL SIGNIFICANCE OF A PROTEASE INHIBITOR, NEWLY ISOLATED FROM ANIMAL TISSUES, IN INFLAMMATION.
Semiannual rept. for 1 Jun-30 Nov 64,
KUMAMOTO UNIV (JAPAN) MEDICAL SCHOOL
Pagination or Media Count:
The present work confirms further physiocochemical properties of a specific protease inhibitor Its molecular weight is 12,500 700 when estimated by the method of Archibald. The isoelectric point of this inhibitor is around 6.6 and its sedimentation coefficient is 1.21S. It gives faint reaction in the biuret test. The substance shows maximal absorbancy at 276 millimicrons and its ratio of absorbancies of 276 and 260 millimicrons is 1.61, showing that it is free of nucleic acid. The inhibitor increases with development of Arthus inflammation, particularly after peak of proteolytic activity of the lesion its peak corresponds to the maximal height of the lesion. Accordingly, the decrease in the protease activity, appearing with development of healing of the lesion, is due to local increase of this inhibitor. The inhibitor forms the complexes with the Arthus protease resulting in its inactivation, but the complexes are present in an easily dissociable state in the healing skin site.
APPROVED FOR PUBLIC RELEASE