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IMMUNOLOGICAL INTERACTION BETWEEN ALLOGENEIC AND XENOGENEIC SPLEEN CELL MIXTURES IN VITRO: STIMULATION OF DNA SYNTHESIS,
NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF
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An early after 24 hours proliferative response measured by 3H-thymidine uptake followed mixing and culture in vitro of spleen cell suspensions derived from certain genetically unrelated rodents. This response reached a maximum value at 40-44 hours. When either component of the mixture in equivalent amounts was cultured alone as a control only a limited response occurred. This marked stimulation of DNA synthesis occurred when spleen cells derived from LAF1 mice and a pool of spleen cells from three noninbred rats were mixed and cultured. No such response occurred in the mouse spleen cultures either, a in the absence of rat spleen cells or, b in the presence of disrupted frozenthawed rat spleen cells. A similar response occurred when spleen cells from one rat were mixed and cultured either c with C57BL10J or DBA2J mouse spleen cells or, to a less marked extent, d with spleen cells from a second unrelated noninbred rat. A similar marked response occurred in such mixtures derived from C57BL10J and DBA2J and from BALBc and C3HHeJ mice. The data, insofar as they now extend, clearly suggest that the response of these rodent spleen cells in the mixed lymphocyte culture MLC test is dependent on certain genetic differences inherent in the two cell populations. Author
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