Accession Number:

AD0625473

Title:

CYTOCHEMISTRY AND BIOCHEMISTRY OF P. BRASILIENSIS.

Descriptive Note:

Annual technical rept. no. 1, 15 Oct 64-15 Oct 65,

Corporate Author:

INSTITUTO VENEZOLANO DE INVESTIGACIONES CIENTIFICAS CARACAS DEPARTAMENTO DE PATOLOGIA EXPERIMENTAL

Personal Author(s):

• Carbonell,Luis M.

Report Date:

1965-11-01

Pagination or Media Count:

17.0

Abstract:

The high amount of intrinsic factors which reduced NBT is a serious chalenge to demonstrate dehydrogenase and diaphorase in the P. brasiliensis fungus. To overcome this difficulty, extraction with acetone and an alkalized buffer was used. With this procedure there was an extraction of several components. It was demonstrated histochemically that succinic acid, DPN, TPN, DPNH and TPNH were extracted. In relation with the intrinsic factor which endogeneously reduced NBT, it was shown that succinic acid played a minor role as endogeneous substrate, while DPNH and TPNH played the main role. The dehydrogenases and diaphorases are located in the mitochondrias, but there is always some reduction of formazan in the cytoplasm. Since the mitochondrias were so well visualized with these techniques, a complete description of them was made. The demonstration of alpha-glycerophosphate dehydrogenase shows that glycerol fermentation is taking place via glicolisis. The presence of L-malic, DL-isocitric and succinic dehydrogenase points out the TCA cicle in the fungus. Demonstration of glucose-6-phosphate dehydrogenase shows that pentose cicle takes place in P. brasiliensis. Author

Descriptors:

• (*FUNGI
• BIOCHEMISTRY)
• (*CYTOCHEMISTRY
• FUNGI)
• IMMUNOLOGY
• TISSUE EXTRACTS
• OXIDOREDUCTASES
• HISTOLOGICAL TECHNIQUES
• MITOCHONDRIA
• CYTOPLASM
• GLYCEROLS
• METABOLISM
• GLYCOGEN
• POLYSACCHARIDES
• YEASTS
• ENZYMES
• CELL STRUCTURE
• VENEZUELA

Subject Categories:

• Biochemistry
• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE