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THE UPTAKE OF GAMMA-AMINOBUTYRIC ACID AND NOREPINEPHRINE BY SUBCELLULAR FRACTIONS OF RAT BRAIN,
CORNELL UNIV ITHACA N Y COGNITIVE SYSTEMS RESEARCH PROGRAM
Pagination or Media Count:
The uptake of two candidate transmitters by subcellular components of neuronal tissue is examined. Gamma aminobutyric acid and norepinephrine, radioactively labeled, were incubated with isolated fractions of brain homogenates and the decrease in the radio-activity of the soluble phase measured. The uptake was determined as a function of the final concentration of the candidate. The effects of drugs on the uptake phenomena were also determined. The uptake of GABA seems to be due to a rapid binding to an external site on the nerve ending particle, followed by a binding to a series of internal sites. The uptake of norepinephrine is due to at least three distinct phenomena. These can be partially separated by density gradient centrifugation and appear to be associated with microsomes, nerve endings, and either mitochondria or a more dense population of nerve endings. Methods for the study of the uptake of GABA and norepinephrine have been established and the effects of certain procedural modifications have been determined. The basic characteristics of the uptake of these two substances have also been determined, and it has been shown that in both cases the phenomenon is a multi-phasic process which must be studied systematically. In addition, preliminary data has been provided on the activation and inhibition of the uptake by various drugs.
APPROVED FOR PUBLIC RELEASE