Accession Number:
AD0621814
Title:
THIN-LAYER CHROMATOGRAPHY OF SOME 131-I AND 125-I LABELLED IODOPYRIMIDINES AND RELATED NUCLEOSIDES,
Descriptive Note:
Corporate Author:
SOCIETA RICERCHE IMPIANTI NUCLEARI SALUGGIA (ITALY) CENTRO RICERCHE NUCLEARI
Personal Author(s):
Report Date:
1964-06-22
Pagination or Media Count:
7.0
Abstract:
The separation of some radioiodinated pyrimidines and nucleosides of biological interest was studied by thin-layer chromatography. Rf values of iodouracil, iodocytosine, iodouridine, iododeoxyuridine and iodocytidine on layers of silica gel G and cellulose G were measured for a number of solvents n-butanol saturated with water, water, n-butanol saturated with 0.1 N H3BO3, n-butanol saturated with i N H3BO3, a saturated solution of H3BO3 in water, 0.1 N HCOOH, n-butanol saturated with 0.1 N HCOOH and n-butanol saturated with o.i N NH4OH were used. With a proper choice of elution system and support, a very good resolution of the single spots can be attained and the relative percentages of the labelled components in a sample can be evaluated with a negligible error by radioactive scanning of the chromatographic plate. Author