Accession Number:

AD0621794

Title:

PROTEINS RADIOIODINATION BY AN ELECTROLYTIC TECHNIQUE,

Descriptive Note:

Corporate Author:

SOCIETA RICERCHE IMPIANTI NUCLEARI SALUGGIA (ITALY) CENTRO RICERCHE NUCLEARI

Report Date:

1965-01-01

Pagination or Media Count:

17.0

Abstract:

An electrolytical method is proposed as a general tool for radioiodine labelling of proteins elemental iodine is set free at the anode by electrolysis of a KI solution in which a protein is dissolved. Preliminary experiments carried out on the amino acids chiefly involved in protein iodination proved that for a proper value of current i.e. a proper value of elemental iodine in the solution, unwanted oxidative reactions can be minimized. A detailed investigation was carried out on the factors affecting the electrolytic process of human serum-albumin iodination. Electrophoresis and microimmuno electrophoresis on agar were adopted to check possible denaturation occurring in the proteins during the iodination process. The results obtained prove that a human serum albumin labelled with I131 at a sufficient specific activity may be produced with a 90 iodination yield and radioiodine content less than 1 atom protein molecule. Results about gamma globulin, fibrinogen and insulin iodination are also reported. I131 labelled albumin and gamma globulin were used for turnover studies in normal man, using a whole body counting system. The results obtained are briefly reviewed, and critically analyzed from the point of view of the adequacy of the tracer for protein turnover experiments. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE