Accession Number:
AD0621794
Title:
PROTEINS RADIOIODINATION BY AN ELECTROLYTIC TECHNIQUE,
Descriptive Note:
Corporate Author:
SOCIETA RICERCHE IMPIANTI NUCLEARI SALUGGIA (ITALY) CENTRO RICERCHE NUCLEARI
Personal Author(s):
Report Date:
1965-01-01
Pagination or Media Count:
17.0
Abstract:
An electrolytical method is proposed as a general tool for radioiodine labelling of proteins elemental iodine is set free at the anode by electrolysis of a KI solution in which a protein is dissolved. Preliminary experiments carried out on the amino acids chiefly involved in protein iodination proved that for a proper value of current i.e. a proper value of elemental iodine in the solution, unwanted oxidative reactions can be minimized. A detailed investigation was carried out on the factors affecting the electrolytic process of human serum-albumin iodination. Electrophoresis and microimmuno electrophoresis on agar were adopted to check possible denaturation occurring in the proteins during the iodination process. The results obtained prove that a human serum albumin labelled with I131 at a sufficient specific activity may be produced with a 90 iodination yield and radioiodine content less than 1 atom protein molecule. Results about gamma globulin, fibrinogen and insulin iodination are also reported. I131 labelled albumin and gamma globulin were used for turnover studies in normal man, using a whole body counting system. The results obtained are briefly reviewed, and critically analyzed from the point of view of the adequacy of the tracer for protein turnover experiments. Author