Accession Number:

AD0621787

Title:

LABELLING OF HUMAN FIBRINOGEN WITH IODINE131 BY ELECTROLYTIC IODINATION,

Descriptive Note:

Corporate Author:

SOCIETA RICERCHE IMPIANTI NUCLEARI SALUGGIA (ITALY) CENTRO RICERCHE NUCLEARI

Report Date:

1963-09-26

Pagination or Media Count:

9.0

Abstract:

An original method for labelling proteins with radioactive I is proposed, and its application to the 131I iodination of human fibrinogen is illustrated. The method is based on the electrolysis of a solution of K131I in presence of the protein to be labelled. The labelling procedure and the electrolytic cell are described in detail. The advantages in comparison with the traditional techniques are 3-fold a there is no need of oxidizing agents b the possibility exists of controlling the rate of liberation of I and c there is the possibility of using extremely dilute solutions of KI, thus reducing the amount of I introduced into the molecule for a given specific activity. The product thus obtained does not show modifications of its original characteristics, as judged from electrophoresis in agar and from the values of the turnover-time which are obtained in a group of normal human subjects. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE