Accession Number:

AD0621528

Title:

5-HYDROXYTRYPTAMINE RELEASE BY BRAIN SLICES.

Descriptive Note:

Technical rept. for Sep 61-Jul 63,

Corporate Author:

CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD

Report Date:

1965-06-01

Pagination or Media Count:

18.0

Abstract:

Brain slices from the rat were allowed to respire for 2 hr in a modified Tyrodes solution containing the monoamine oxidase inhibitor 1-phenyl-2-hydrazinopropane alpha-methyl phenyl hydrazine, JB-516. The filtered Tyrodes solution was shown to contain 5-hydroxytryptamine Serotonin, 5-HT by both bioassay and fluorometric techniques. Preliminary evidence indicates that the amount of 5-HT in the filtrate is increased significantly by adding reserpine and JB-516 to the incubation medium. These findings suggest that a sensitive bioassay procedure, coupled with the use of brain slices. offers an approach for the study of factors affecting the storage and release of endogenous 5-HT. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE