Accession Number:

AD0621390

Title:

INHIBITION OF GUINEA PIG C'2 BY RABBIT ANTIBODY. QUANTITATIVE MEASUREMENT OF INHIBITION, DISCRIMINATION BETWEEN IMMUNE INHIBITION AND COMPLEMENT FIXATION, SPECIFICITY OF INHIBITION AND DEMONSTRATION OF UPTAKE OF C'2 BY EAC'LA,4,

Descriptive Note:

Corporate Author:

JOHNS HOPKINS UNIV BALTIMORE MD DEPT OF MICROBIOLOGY

Personal Author(s):

Report Date:

1964-09-23

Pagination or Media Count:

23.0

Abstract:

On repeated inoculation with alum-precipitated, partially purified guinea pig C2, rabbits form antibody which inhibits the hemolytic activity of C2. A sensitive method has been designed for precise quantitative measurement of inhibiting anitbody. The procedure excludes the possibility of complement fixation and, hence, the inhibitory effect is attributed to steric hindrance. The shape of the anti-C2 neutralization curve indicates that a single molecule of antibody suffices for inhibition of C2, and that the C2 molecule can bind several molecules of neutralizing antibody. Quantitative inhibition analyses indicate that C2 physically combines with EACla,4 to yield the intermediate EACla,4,2a. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE