Accession Number:

AD0621111

Title:

STAINING OF VACCINIA ANTIGEN BY IMMUNOURANIUM TECHNIQUE,

Descriptive Note:

Corporate Author:

CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD

Report Date:

1964-10-10

Pagination or Media Count:

11.0

Abstract:

An attempt to follow morphologically the development of vaccinia antigen in helium-lanthanum HeLa cells is reported. The conversion of rabbit antisera to vaccinia virus and the preparation of vaccinia-infected HeLa cells for electron microscopy are described. With specific staining, viral particles were found 14 hr after infection. They were characterized by wall staining and, in most instances, absence of core staining. Two types of localization were observed. In one type, the particles were scattered in small clusters in the cytoplasm. The remaining sections of cytoplasm contained large, specifically stained masses consisting of closely packed, viral particles and amorphous stained material. The former type of localization also showed staining in areas beyond the confines of viral particles that was not noted in control sections. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE