Accession Number:

AD0620977

Title:

ZONE ELECTROPHORESIS OF HUMAN PAROTID SALIVA IN ACRYLAMIDE GEL,

Descriptive Note:

Corporate Author:

NAVAL DENTAL RESEARCH INST GREAT LAKES ILL

Personal Author(s):

Report Date:

1965-03-01

Pagination or Media Count:

5.0

Abstract:

The report concerns an examination of acrylamide gel as another medium for the zone electrophoresis of parotid saliva proteins. Preliminary experiments with acrylamide-gel strips yielded protein patterns in which twenty or more fractions could be visually distinguished when the electrophoresis was carried out in 0.1 M tris-EDTAboric acid buffer at pH 9.0. Under the conditions of the experiment, more than half the staining components migrated anodically with generally better resolution than is evident for the cathodically-migrating components. Salivary amylase, a troublesome factor in the starch-gel electrophoresis of saliva proteins, does not attack acrylamide gel.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE