ZONE ELECTROPHORESIS OF HUMAN PAROTID SALIVA IN ACRYLAMIDE GEL,
NAVAL DENTAL RESEARCH INST GREAT LAKES ILL
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The report concerns an examination of acrylamide gel as another medium for the zone electrophoresis of parotid saliva proteins. Preliminary experiments with acrylamide-gel strips yielded protein patterns in which twenty or more fractions could be visually distinguished when the electrophoresis was carried out in 0.1 M tris-EDTAboric acid buffer at pH 9.0. Under the conditions of the experiment, more than half the staining components migrated anodically with generally better resolution than is evident for the cathodically-migrating components. Salivary amylase, a troublesome factor in the starch-gel electrophoresis of saliva proteins, does not attack acrylamide gel.