ENCAPSULATION OF VIRUSES
Rept. for Mar-Oct 1964
NATIONAL CASH REGISTER CO DAYTON OH CAPSULAR PRODUCTS RESEARCH AND DEVELOPMENT
Pagination or Media Count:
Research efforts were directed toward development of a method of encapsulating viruses in spheres containing a predictable phage titer to be used in biological dosimetry experiments. Initial studies demonstrated the ability of the bacteriophage to undergo lyophilization and encapsulation yielding a product of adequate viability. The Butarez Toluene Ethylcellulose encapsulation system proved satisfactory for producing spheroidal virusethylcellulose capsules of twenty-five to fifty microns diameter, the mean diameter being forty-two microns. Viability determinations yielded a phage titer of 1.7 x 10 to the 11th power phages per gram of capsules hence, a capsule of average diameter possessed a theoretical titer of 8 x 1000 phages. Simulated end-use tests proved photographic developer chemicals, such as could be used for the processing of virus-bearing nuclear emulsions, to be nontoxic to the encapsulated phages. A modification in the scope of the program directed the concluding work to the preparation of capsules less than fifteen microns in diameter. Samples of virusethylcellulose capsules were submitted to the Air Force.