Accession Number:

AD0620716

Title:

PURIFICATION AND N-TERMINAL ANALYSES OF ALGAL BILIPROTEINS,

Descriptive Note:

Corporate Author:

UNIVERSITY COLL GALWAY (IRELAND)

Personal Author(s):

Report Date:

1964-05-19

Pagination or Media Count:

6.0

Abstract:

R-, B- and C-phycoerythrins and R- and C-phycocyanins were isolated and purified on a preparative scale by calcium phosphate chromatography, ammonium sulphate fractionation and crystallization. The N-terminal residues of these biliproteins were analysed. Methionine is the only N-terminal residue of all the phycoerythrins, there being about 14 N-terminal residues per molecule of Rand B-phycoerythrins mol.wt. 290 000 and about 8 per molecule of C-phycoerythrin mol.wt. 226 000. Threonine 1 residue is N-terminal in C-phycocyanin mol.wt. 138 000, and both threonine about 1.3 residues and methionine 5 residues are N-terminal in R-phycocyanin mol.wt. 273 000. Results suggest that the apoproteins of the various phycoerythrins are closely related, whereas C-phycocyanin has quite a different gross structure, and that R-phycocyanin contains two types of sub-unit, one related to C-phycocyanin and the other to the phycoerythrins. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE