Accession Number:

AD0620629

Title:

ISOLATION OF COAGULANT AND ANTICOAGULANT PRINCIPLES FROM THE VENOM OF TRIMERESURUS GRAMINEUS,

Descriptive Note:

Corporate Author:

NATIONAL TAIWAN UNIV TAIPEI COLL OF MEDICINE

Personal Author(s):

Report Date:

1965-01-01

Pagination or Media Count:

9.0

Abstract:

The venom of Trimeresurus gramineus was separated into five fractions, namely alpha, beta, gamma, delta, and epsilon by means of zone electrophoresis on starch with borate-NaCl buffer pH 7.6, and ionic strength 0.062. Only the alpha-fraction migrated towards the anode. The beta-fraction remained at the position of application and the other three fractions migrated towards the cathode. The highest thrombin-like and TAMe-esterase activities were located next to the peak of the alpha-fraction on the anode side. Both activities were about seven times stronger than those of the crude venom. The proteolytic activity of the alpha-fraction was less than one half that of the crude venom. The beta-fraction showed only feeble thrombinlike, TAMe-esterase and proteolytic activities. The gamma-fraction was the principal portion with anticoagulant as well as proteolytic activities. The delta and epsilon-fractions had little effect on blood coagulation, although a moderate proteolytic activity was present in the delta-fraction. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE