ANTIGENIC PRIPERTIES OF TYPE E CL. BOTULINUM PROTOXIN
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
In the tests with twelve strains of C1. botulinum type E it was established that in liquid nutrient media this microbe produces a toxin and a protoxin. The latter converts into a toxin under the action of several enzymes. The titer of the toxin following activation was considerably increased, and the index of activation fluctuated within the limits of 10 up to 1,000. For the neutralization of the same volumes of native toxin of type E before and after activation by enzymes the same amount of antitoxic units was required, while the lethal activity of the toxin following activation increased by 10 to 100 times, i.e., the antigenic properties of the protoxin were equivalent to the antigenic properties of the toxin. With the type E botulinum toxin before and after activation an indirect hemagglutination reaction was obtained in the same dilutions, in spite of the fact that the activity of the toxin following activation was increased by many times, which also testified to the equivalence of the protoxin and the toxin. The data obtained when studying the antitoxin binding capability of the toxins and anatoxins of type E also testified to the presence of antigenic properties in the protoxins of type E. The anatoxins obtained from botulinum toxins type E both of non-activated as well as of toxins activated by pancreatin or C1. sporogenes, possessed the same antitoxin binding properties when the reaction was set up on white mice.