THE EFFECT OF THE TEMPERATURE REGIMEN USED DURING CULTIVATION ON THE CHEMICAL COMPOSITION OF PASTEURELLA PESTIS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
A difference was noted in the chemical composition of Past. pestis cultured on Hottingers agar at 28 and 37C. As demonstrated, Past. pestis cells grown at 37C differed from those grown at 28C by a considerably increased polysaccharide content, a greater amount of lipids and a lesser amount of glucosamine and phosphorus, mainly of the RNA phosphorus. Galactose, glucose, xylose and ribose were revealed in the polysaccharide portion of both cultures. The galactose content was much greater in cultures grown at 37C than in those grown at 28C, whereas the glucose and ribose content was about double in cultures grown at 28C. As established, polysaccharide, accumulated in considerable quantities by Past. pestis, grown at 37C, was nonspecific. On the basis of the data obtained a supposition was put forward on the relationship between the capacity of Past. pestis, grown at 37C, to nonspecific polysaccharide accumulation and the synthesis of specific capsular protein in these conditions.