PROTEIN AND POLYSACCHARIDE DISTRIBUTION IN P. PESTIS GROWN AT 28 DEGREES AND 37 DEGREES
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The cultivation of the plague microbe on an agar medium at 37 degrees characterized by the increased formation of capsule under these conditions, led to an increase in the amount of easily extracted protein components, while at the same time the total content of protein in cells incubated at 28 and 37 degrees remained constant. A change of incubation temperature did not show up in the extraction ability of the polysaccharide components of the cell. Regardless of the temperature of incubation of the culture, the polysaccharide which contained arabinose passed into the extract with considerable ease, and the polysaccharide containing glucose -- with much difficulty. This supports the supposition of the existence in the plague microbe of two specific carbohydrate components The capsular -- containing arabinose, and the somatic -- containing glucose.