Accession Number:

AD0619786

Title:

QUANTITATION OF DELAYED HYPERSENSITIVITY IN GUINEA-PIGS BY A LOCAL PASSIVE TRANSFER REACTION,

Descriptive Note:

Corporate Author:

ILLINOIS UNIV URBANA COLL OF VETERINARY MEDICINE

Personal Author(s):

Report Date:

1965-01-01

Pagination or Media Count:

22.0

Abstract:

Since peritoneal exudate cells from sensitive guinea pigs are capable of conferring delayed hypersensitivity DH onto recipient guinea pigs, it should be possible to measure the degree of sensitivity of the donor by finding the minimal number of cells capable of transferring DH to a recipient. The systemic passive transfer of DH, however, often requires several donors to sensitize a single recipient. Local passive transfer of DH was accomplished by intradermal injection of sensitive peritoneal exudate cells, followed by intracardial challenge of the recipients with antigen. Erythema and induration developed at the sites of injection of sensitive cells within 6 to 8 h after challenge and persisted for 12 to 18 h. Titration of DH was accomplished by injecting different numbers of donors cells at separate skin sites of the same recipient and finding the minimal number of cells capable of transferring local DH. As few as 160,000 peritoneal exudate cells transferred local DH in some trials. The development of DH was studied as a function of time. Delayed hypersensitivity was first detected on the sixth day after sensitization of a group of cell donors and reached a peak on the eighth day. The findings agreed with results of skin testing of actively sensitized guinea pigs. The technique of local passive transfer appears adequate for quantitative study of DH. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE