IMPROVEMENT OF THE NUTRIENT MEDIUM AND THE SEARCH FOR A METHOD OF PURIFYING THE PROTECTIVE ANTHRAX ANTIGEN
ARMY BIOLOGICAL LABS FREDERICK MD
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As a result of work on cultivation of anthrax microbe to obtain a protective antigen a milk-peptone medium was suggested. To isolate the anthrax antigen from the culture fluid a method of its precipitation with ethyl alcohol is recommended, with subsequent concentration and stabilization of the antigen, its sorption on aluminum hydroxide and lyophilization. Chemical sorbed vaccine, obtained by the mentioned method, provoked but insignificant postvaccinal reactions, and possessed sufficiently high immunogenic properties.