DETECTION OF BOTULIN TOXIN IN CANNED FOOD WITH THE HELP OF THE INDIRECT HEMAGGLUTINATION REACTION AFTER BOYDEN,
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The indirect hemagglutination reaction after Boyden with Konikovas modification was used with the aim of detecting botulin toxin in canned foods. Based on the results of the biological test, the activity of botulin toxin in the contaminated cans reached 1600-3200 Dlm in 1 ml for type A toxin and 320-640 Dlm in 1 ml for type B toxin. A conclusion concerning the presence of toxin may be made in 12-24 hours and concerning the degree of activity in 3-4 days. By using the indirect hemagglutination reaction in these tests it was possible to expose 16-164 Dlm of toxin in 1 ml in 1-112 hours after setting up the experiment. The method was considerably more sensitive and made it possible to obtain the results faster than by the biological test.