STABILIZATION OF THE IMMUNOGENIC PROPERTIES OF THE GIRARD AND ROBIK EV STRAINS
ARMY BIOLOGICAL LABS FREDERICK MD
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Conclusion Repeated passage through guinea pig conjunctiva or subcutaneous and intraperitoneal administration of diminishing doses of EV strain culture provided for enhancement and stabilization of the immunogenicity of the strain. The passage methods used did not lead to more intense virulency and reactogenicity of the EV strain experiments in animals and pathohistological investigations confirmed its initial innocuousness. In undertaking passages a culture isolated from a guinea pig sacrificed not later than the fifth to sixth day after inoculation should be used. Prolonged residence of the vaccine culture in the animal organism led to a significant decrease in its immunogenicity. Cultures obtained from individual colonies of the same strain, not differing in form, is different in immunogenicity. The method of studying individual colonies can be employed for selecting immunogenicity of EV strain subcultures.