Accession Number:

AD0618857

Title:

THE BIOSYNTHESIS OF BIOTIN IN MICROORGANISMS. III. THE BIOSYNTHESIS OF (+)-BIOTIN FROM DESTHIOBIOTIN AND ITS CONTROL IN ESCHERICHIA COLI,

Descriptive Note:

Corporate Author:

CINCINNATI UNIV OHIO COLL OF MEDICINE

Personal Author(s):

Report Date:

1964-07-17

Pagination or Media Count:

7.0

Abstract:

The conversion of desthiobiotin to -biotin by Escherichia coli has been studied. Desthiobiotin is converted to -biotin, as identified by bioautography by both growing and resting cells of a biotinless mutant strain of E. coli. The conversion is also demonstrable in resting cells of a wild-type strain of E. coli. No converting activity was found in either the mutant or wild-type strains which had been grown in the presence of exogenous -biotin. Oxybiotin and desthiobiotin did not cause such repression. The results are in agreement with previous findings that biotin synthesis from glucose is repressed by -biotin, and supports the role of this converting step as the functioning biosynthetic route to the vitamin. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE