STUDIES ON GANGLIOSIDES FROM HUMAN AND CALF BRAIN.
OHIO STATE UNIV COLUMBUS
Pagination or Media Count:
Attempts to characterize the individual gangliosides by component molar ratios have indicated a requirement for reliable glucose and galactose assays at the submilligram level. An investigation was undertaken to develop analytical procedures to meet these requirements. The study resulted in the development of a new gas-liquid chromatographic method which was suitable for the simultaneous quantitative analysis of glucose and galactose from sub-milligram amounts of ganglioside. When this GLC assay was used to characterize a preparation which appeared to be an unreported ganglioside, the data suggested that it was a mixture even though the TLC assay of the preparation indicated that it was homogeneous. Subsequently, it appeared from a study of the most recent literature that no single TLC solvent system was adequate to distinguish the numerous ganglioside species which were reported. Therefore, it was decided to investigate the TLC properties of all the available ganglioside preparations in four commonly used solvent systems. The data are compared to those reported in the literature. It is anticipated that these studies will result in a more complete understanding of the chamical nature of the individual gangliosides.