Accession Number:

AD0618263

Title:

BOUND WATER, INOSITOL, AND THE EFFECT OF X-RAYS ON ESCHERICHIA COLI,

Descriptive Note:

Corporate Author:

SASKATCHEWAN UNIV SASKATOON DEPT OF BACTERIOLOGY

Personal Author(s):

Report Date:

1964-05-11

Pagination or Media Count:

8.0

Abstract:

Aerosols of Escherichia coli B were subjected to 250 kv X-rays. It was found that maximal X-ray damage occurred at 70 to 80 relative humidity R.H.. At these R.H. values only the water bound directly to cell macromolecules remains, and if the water layers were increased by using higher humidities, X-ray damage decreased. Also, at R.H. levels below 70 a sharp decrease in the sensitivity of the cells to the radiation occurred. Several chemicals known to protect cells against desiccation, ultraviolet, and X-ray damage were examined and of these i-inositol proved the most successful. The difference in the protective ability of these various compounds indicated that some protect cells against desiccation damage by retaining water, others by replacing bound-water molecules in macromolecular structure but those retaining water will not protect against X-rays. The results suggest that the physical removal or ionization of a strategic bound-water molecule by X-rays causes most of the cell deaths rather than ionizations occurring in the free water as the presence of the latter appears to offer cells a measure of protection. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE