Accession Number:

AD0617990

Title:

FINE CONTROL OF PHOSPHOPYRUVATE CARBOXYLASE ACTIVITY IN ESCHERICHIA COLI,

Descriptive Note:

Corporate Author:

LEICESTER UNIV (ENGLAND)

Personal Author(s):

Report Date:

1964-12-07

Pagination or Media Count:

4.0

Abstract:

Previous studies with mutants of Salmonella typhimurium and Escherichia coli, which fail to grow on glucose-salts media unless such media are supplemented with intermediates of the tricarboxylic acid cycle or direct precursors thereof, have shown that the carboxylation of phosphoenolpyruvate CH2C.COOH.O.PO3H2 CO2 yields HOOC.CH2.CO.COOH Pi is necessarily involved in the maintenance of the tricarboxylic acid cycle this reaction serves to replenish the cycle with C4-compounds, as intermediates of that cycle are revoved in the course of biosynthesis. It was difficult to explain why CoASAc and citrate synthase should be so much more efficient a trapping agent for labelled oxaloacetate than was malate dehydrogenase and NADH2, even when the extracts were supplemented with crystalline malate dehydrogenase this observation suggested that CoASAc played a role in this system additional to its function as a trapping agent. This suggestion was confirmed with a mutant AB1623 of E. coli K-12.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE