Accession Number:

AD0617983

Title:

UPTAKE OF L- AND D-ISOMERS OF CATECHOLAMINES IN ADRENERGIC NERVE GRANULES,

Descriptive Note:

Corporate Author:

KAROLINSKA INSTITUTET STOCKHOLM (SWEDEN)

Personal Author(s):

Report Date:

1963-06-28

Pagination or Media Count:

6.0

Abstract:

The spontaneous depletion of endogenous noradrenaline NA from bovine splenic nerve granules is diminished or prevented by incubation with either L-NA or D-NA. However, the effect observed following the addition of L-NA is significantly greater than that produced by the D-isomer. No significant difference is observed between the uptake of the two isomers of adrenaline A in undepleted granules. The enhanced uptake of A or NA is a concentration of 10 micro-gml in the presence of adenosine triphosphate ATP similarly did not differ for the L- and D-isomers of the amine. However, the ATP-dependent uptake at an amine concentration of 1 micro-gml was 2-3 times as high for L-NA and L-A than for the D-isomers. It is concluded that the ATP-dependent uptake in nerve granules of NA and A at low amine concentrations is to some extent stereospecific, while the amine-uptake at higher concentrations with and without added ATP is not or only slightly dependent on the steric configuration. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE