Accession Number:

AD0614396

Title:

APPROXIMATE ENZYMIC METHOD OF MICROANALYSIS FOR IDENTIFYING RESIDUAL AMOUNTS OF ORGANOPHOSPHORUS INSECTICIDES,

Descriptive Note:

Corporate Author:

FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO

Personal Author(s):

Report Date:

1965-03-30

Pagination or Media Count:

17.0

Abstract:

A simple, highly sensitive group method of detecting phosphorus-containing insecticides in various objects of the surroundings, based on their inherent anticholinesterase action, was developed. In order to realize this method a portable kit was constructed which contains a rack for the storage of ampouled reagents. The kit contains enough reagents for 100 analyses. The detection of insecticides may be performed under field conditions. A preliminary determination of insecticide contamination of products may be obtained in 10 - 20 min. The anticholinesterase effect of 6 of the most important insecticides was studied and calibration curves were constructed for their determination. The method without preliminary activation makes it possible to determine from 1 to 100 micrograms of insecticide per 1 ml. It was established that the anticholinesterase activity of chlorphos when dissolved in buffer solutions of pH 7.4 and under the action of plant extracts gradually increases. This is evidently explained by a conversion of the insecticide into a compound possessing high anticholinesterase activity. Author

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE