UNBALANCED GROWTH AND LATENT KILLING OF ESCHERICHIA COLI FOLLOWING EXPOSURE TO SULFUR MUSTARD.
Rept. for Feb 62-Mar 64,
CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD
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This report describes the effects of unbalanced metabolism on the survival of strains of Escherichia coli treated with sulfur mustard. Strains studied required thymine, arginine, and uracil 15T-A-U- and thymine and phenylaniline 15T-PA-. Resistant bacteria incubated for 2 hr in a T, -PA medium became progressively sensitive to sulfur mustard when transferred to a -T, -PA medium. Return to their original medium restored their original resistance. Resistant cells preincubated in T, -AU showed higher survivals and were less susceptible to latent killing on post-treatment incubation in a -T, -AU medium. Controls in this medium remained immune to the induction of thymineless death, while mustard-treated cells showed small, dose-dependent, latent-killing effects. Pretreatment in thymidine media gave highest resistances and immunities to thymineless death. Resistant state was developed by subjecting mustard-treated cells to low levels of unbalanced metabolism. Unbalanced metabolism effects DNA and RNA stability. The monofunctional analog, beta-chloroethyl beta-hydroxyethyl sulfide, does not inhibit DNA synthesis nor promote its breakdown. Cross-alkylating reactions are effective for initiating unbalanced metabolism. Author