Accession Number:

AD0614267

Title:

ELIMINATION OF SULFUR MUSTARD-INDUCED ALKYLATION PRODUCTS FROM DNA OF ESCHERICHIA COLI,

Descriptive Note:

Corporate Author:

CHEMICAL RESEARCH AND DEVELOPMENT LABS EDGEWOOD ARSENAL MD

Report Date:

1965-02-01

Pagination or Media Count:

24.0

Abstract:

The objectives of this experiment were to determine if bacteria posses a mechanism for eliminating sulfur mustard-induced damages to their deoxyribonucleic acid DNA and to ascertain the role of such a repair process in the recovery of cells from the effects of radiomimetic alkylating agents. This work is part of a continuing program that is designed to establish a rational approach for therapy against lethal mustard intoxications. Evidence is presented that 1 sulfur mustard-treated E. coli 15T-A-U- deploymerize a significant portion of their DNA during post-treatment metabolism, 2 the rate of loss of sulfur mustard products initially bound to DNA is more rapid than the rate of overall DNA degradation, 3 several sulfur mustardDNA alkylation products including monofunctionally and bifunctionally alkylated guanine are eliminated to equal extents, and 4 sulfur mustard-treated bacteria after a lag recover the capacity to synthesize DNA, suggestive of a causal relationship between the excision of alkylation products from DNA and resumption of DNA replication. Because these bacteria are also capable of excising ultraviolet light-induced thymine dimers, the current results suggest that cells possess a general mechanism for repairing different types of genetic defects. Author

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Distribution Statement:

APPROVED FOR PUBLIC RELEASE