ATROPINE DETOXICATION DURING HYPOTHERMIA BY THE ISOLATED PERFUSED RAT LIVER.
Final technical rept.,
PITTSBURGH UNIV PA SCHOOL OF MEDICINE
Pagination or Media Count:
Experiments were performed on the isolated perfused rat liver in an attempt to obtain a perfusing medium which would be devoid of plasma protein. It was considered that if such a medium could be perfected, it would overcome any hindrance to drug uptake by the liver which might occur due to plasma binding of the drug. Results of this experimentation are outlined. Several exploratory type experiments were performed on the isolated liver in which blood flow through the organ was maintained constant by the readjustment of vascular resistance of the arterialized portal vein. In preparation for a contemplated Air Force Grant request in which the correlation of the toxicity of atropine with its metabolism is acute hypothermia and in cold acclimatization will be studied, work was attempted on a surgical technique which would allow a chronically prepared rat to be intravenously injected and have bile collected without the use of anesthesia.